Bismark

Bismark Processing Report

/sibcb2/bioinformatics/JupyterLab/cromwell-executions/bismarkRRBS/5710a589-9ffe-44c6-892f-07ed3ba6d2d7/call-trim_galore/execution/trimmed/SRX1869508_R1_val_1.fq.gz and /sibcb2/bioinformatics/JupyterLab/cromwell-executions/bismarkRRBS/5710a589-9ffe-44c6-892f-07ed3ba6d2d7/call-trim_galore/execution/trimmed/SRX1869508_R2_val_2.fq.gz

Data processed at 22:37 on 2020-09-10

Alignment Stats

Sequence pairs analysed in total 165719764
Paired-end alignments with a unique best hit 111389612
Pairs without alignments under any condition 25396726
Pairs that did not map uniquely 28933426
Genomic sequence context not extractable (edges of chromosomes) 5

Cytosine Methylation

Total C's analysed 5040845322
Methylated C's in CpG context 356622058
Methylated C's in CHG context 1690649
Methylated C's in CHH context 2741435
Methylated C's in Unknown context 9977
Unmethylated C's in CpG context 560249454
Unmethylated C's in CHG context 1292992773
Unmethylated C's in CHH context 2826548953
Unmethylated C's in Unknown context 509624
Percentage methylation (CpG context) 38.9%
Percentage methylation (CHG context) 0.1%
Percentage methylation (CHH context) 0.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 55464412 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 55925195 original bottom strand

Cytosine Methylation after Extraction

Total C's analysed 3260249111
Methylated C's in CpG context 251119312
Methylated C's in CHG context 1216342
Methylated C's in CHH context 1773794
Unmethylated C's in CpG context 356563189
Unmethylated C's in CHG context 815223712
Unmethylated C's in CHH context 1834352762
Percentage methylation (CpG context) 41.3%
Percentage methylation (CHG context) 0.1%
Percentage methylation (CHH context) 0.1%

M-Bias Plot